Effect of Moderate UVC Irradiation on Bovine Serum Albumin and Complex with Antimetabolite 5-Fluorouracil: Fluorescence Spectroscopic and Molecular Modelling Studies-Reference-Cited by-同舟云学术

Effect of Moderate UVC Irradiation on Bovine Serum Albumin and Complex with Antimetabolite 5-Fluorouracil: Fluorescence Spectroscopic and Molecular Modelling Studies

Published:2015-01-31 Issue: Volume:2015 Page:1-12
ISSN:1687-9449
Container-title:International Journal of Spectroscopy
language:en
Short-container-title:International Journal of Spectroscopy

Author:

Chinnathambi Shanmugavel¹²,Karthikeyan Subramani³,Velmurugan Devadasan³⁴,Hanagata Nobutaka²⁵,Aruna Prakasarao¹,Ganesan Singaravelu¹

Affiliation:

1. Department of Medical Physics, Anna University, Chennai 600 025, India

2. Nanotechnology Innovation Station, National Institute for Materials Science, 1-2-1 Sengen, Tsukuba, Ibaraki 305-0047, Japan

3. Centre of Advanced Study in Crystallography and Biophysics, University of Madras, Chennai 600 025, India

4. Bioinformatics Infrastructure Facility, University of Madras, Chennai 600 025, India

5. Graduate School of Life Science, Hokkaido University, N10W8 Kita-ku, Sapporo 060-0812, Japan

Abstract

The interaction of antimetabolite 5-fluorouracil (5FU) with bovine serum albumin (BSA) under UVC (253.7 nm) irradiation was investigated in the present study using UV-Vis spectroscopy, steady state/time resolved fluorescence spectroscopic techniques. The stability of protein was found to be very strong when BSA gets bind to 5FU and moreover it is compared with the free BSA under UVC irradiation. From the fluorescence spectroscopic study, the stability of the complex was found to acquire 2-fold stronger than free protein. From the molecular modelling studies, we came to know the hydrogen bonds between BSA and antimetabolite 5FU are strong, up to 70.4 J/m2 under UVC irradiation.

Funder

Department of Atomic Energy, Government of India

Publisher

Hindawi Limited

Subject

Rehabilitation,Physical Therapy, Sports Therapy and Rehabilitation,General Medicine

Link

http://downloads.hindawi.com/archive/2015/315764.pdf

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