Apoptosis Transcriptional Profile Induced byPorphyromonas gingivalisHmuY

Abstract

This study aimed at evaluating the transcriptional profile of apoptosis-related genes afterin vitrostimulation of peripheral blood mononuclear cells (PBMCs) derived from individuals with periodontitis (P) and healthy nonperiodontitis (NP) control subjects withP. gingivalisHmuY protein. PBMCs from the P and NP groups were stimulated with HmuYP. gingivalisprotein, and the expression of genes related to apoptosis was assessed by custom real-time polymerase chain reaction array (Custom RT2PCR Array). Compared with the NP group, the P group showed low relative levels of apoptosis-related gene expression, downregulated for FAS, FAS ligand, TNFSF10 (TRAIL), BAK1, CASP9, and APAF1 afterP. gingivalisHmuY protein stimulation. Furthermore, the P group exhibited low levels of relative gene expression, downregulated for CASP7 when the cells were not stimulated. Our data suggest thatP. gingivalisHmuY protein might participate differently in the modulation of the intrinsic and extrinsic apoptosis pathways.