Antibacterial Activities andIn VitroAnti-Inflammatory (Membrane Stability) Properties of Methanolic Extracts ofGardenia coronariaLeaves

Abstract

This work is carried out withGardenia coronarialeaves that belong to the family Rubiaceae, which is a small-to-medium-sized but tall, deciduous tree, 7.6–9 m high on an average. Leaves are used for the treatment of rheumatic pain and bronchitis. The leaf of the plant consists of coronalolide, coronalolic acid, coronalolide methyl ester, ethyl coronalolate acetate triterpenes (secocycloartanes), and so forth. Methanol extract from the leaves ofGardenia coronariawas completely screened for membrane stability and antibacterial activity. The lower concentrations of Methanolic leaf extract ofGardenia coronariagave good antimicrobial and anti-inflammatory activity, but higher concentrations gave relatively more projecting antibacterial activityin vitroas compared with Kanamycin. The crude drug’s anti-inflammatory effects were compared with those of Aspirin as positive control. The Methanolic extracts ofGardenia coronarialeaves possessed a broad spectrum antibacterial activity against a variety of both Gram-negative and Gram-positive organisms likeStreptococcus agalactiae,Escherichia coli,Pseudomonas aeruginosa,Bacillus cereus,Shigella sonnei,Shigella boydii, andProteus mirabilis, with a zone of inhibition from 10 to 16 mm. The extract also showed good membrane stability to be considered as having significant anti-inflammatory action.