Toxicological Evaluation and Hepatoprotective Efficacy of Rosmarinic Acid-Rich Extract from Ocimum basilicum L.

Abstract

Exposure to carbon tetrachloride (CCl4) induces acute and chronic liver injuries as well as oxidative stress in rats. The present study was designed to evaluate the in vivo toxicity of rosmarinic acid-rich extract from Ocimum basilicum (RAE). The acute and subchronic oral toxicity of RAE was evaluated in Albinos mice. Hepatotoxicity was induced by the administration of CCl4-induced hepatic injury in rats. The hepatoprotective effect of RAE on aspartate aminotransferase, alanine transaminase, alkaline phosphatase, lactate dehydrogenase, bilirubin, total protein, albumin, triglycerides, total cholesterol, low-density lipoprotein, high-density lipoprotein, plasmatic glucose, urea, creatinine, and malondialdehyde was determined in CCl4-intoxicated rat. The extract did not produce treatment-related signs of toxicity or mortality in any of the animals tested during acute as well as subchronic toxicity studies. The administration of CCl4 resulted in marked increase in plasma hepatic enzymes $\left(p<0.001\right)$ and significant decrease of total protein $\left(p<0.001\right)$ and albumin $\left(p<0.001\right)$ when compared to normal. The RAE at 200 mg/kg body weight lowered significantly $\left(p<0.001\right)$ plasma enzyme activities of liver, which is designation of hepatoprotective action of extract. The phenolic extract exerts a significant increase in total protein $\left(p<0.001\right)$ , and albumin $\left(p<0.001\right)$ , accompanied with a marked reduction in the levels of malondialdehyde $\left(p<0.001\right)$ , as compared to CCl4-treated group. Our study suggests that RAE may be used as a hepatoprotective agent against toxic effects caused by CCl4 and other chemical agents in the liver.