Structural characterization of recombinant bovine Goαby spectroscopy and homology modeling

Abstract

Go, a member of heterotrimeric guanine nucleotide-binding proteins, is the most abundant form of G protein in the central and peripheral nervous systems. Goαhas a significant role in neuronal development and function but its signal transduction mechanism remains to be clarified. In this study, the bovine Goαsubunit was overexpressed and purified into homogeneity. Its activity was studied using [35S] GTPγS binding, intrinsic fluorescence and BODIPY assays. The secondary structure was determined by both FTIR and CD spectroscopy as 42.3%α-helix, 13.4%β-sheet and 24.3%β-turn. A theoretical structure model was constructed. The structure from homology modeling is in very good agreement with the crystal structure of mouse Goαsubunit except for the loop betweenαB–αC helices. This model was docked to the mouse RGS16 molecule. T117 on theαB–αC loop of Goαinteracted with K172 on RGS16 as opposed to the T117 and K164 interaction in mouse.