Vaginal Microbiota of Healthy Pregnant Mexican Women is Constituted by Four Lactobacillus Species and Several Vaginosis-Associated Bacteria

Abstract

Objective. To identify the microbiota communities in the vaginal tracts of healthy Mexican women across the pregnancy.Methods. Vaginal swabs were obtained during the prenatal visit of women from all trimesters () of healthy pregnant women of Mexico City. DNA was isolated from each sample, and PCR-DGGE and sequencing of 16S rRNA gene fragments were used to identify the bacterial communities.Results. 21 different microorganisms were identified in the vaginal samples.Lactobacillusgenus was present in 98% of women studied. Four lactobacilli species were identified in vaginal samples.L. acidophiluswas the predominant (78%) followed byL. iners(54%),L. gasseri(20%), andL. delbrueckii(6%). 17 different microorganisms related to bacterial vaginosis conditions were identified.Ureaplasma urealyticumwas the predominant (21%) followed by BVAB1 (17%) andGemella bergeriae(7.8%).Conclusions.Lactobacillusgenus predominates in the vaginal samples of Mexican pregnant women associated with different microorganisms related to bacterial vaginosis conditions.