TMEM25Is a Candidate Biomarker Methylated and Down-Regulated in Colorectal Cancer

Author:

Hrašovec Sonja1,Hauptman Nina1,Glavač Damjan1,Jelenc Franc2,Ravnik-Glavač Metka13

Affiliation:

1. Department of Molecular Genetics, Institute of Pathology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia

2. Department of Abdominal Surgery, University Medical Centre Ljubljana, Ljubljana, Slovenia

3. Institute of Biochemistry, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia

Abstract

The identification of novel genes involved in colorectal cancerogenesis is of high clinical relevance for early diagnosis, applying new therapeutic strategies and monitoring disease recurrence, in order to reduce disease incidence and mortality. Gene silencing through CpG island hypermethylation is a major epigenetic mechanism involved in cancer development. In our study, we aimed to identify and validate novel genes with a tumour specific DNA methylation profile in colorectal cancer. We performed a whole-genome methylation scan and identified several possible candidate genes that are hypermethylated in tumour in comparison to healthy colon mucosa. Using methylation-specific high-resolution melting analysis in a set consisting of 133 colorectal cancer samples, we were able to confirm an altered CpG site inTMEM25in 69.2% (92/133) tumours analysed. Furthermore, the expression ofTMEM25was found to be significantly lower in tumour tissue. An inverse correlation between hypermethylation ofTMEM25andTMEM25down-regulated expression was observed.Our results suggest that epigenetic down-regulation ofTMEM25is cancer-related; we thus suggest thatTMEM25hypermethylation might play a significant role in altering expression of this gene in colorectal cancer.

Publisher

Hindawi Limited

Subject

Biochemistry, medical,Clinical Biochemistry,Genetics,Molecular Biology,General Medicine

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3