Cauliflower Mosaic Virus TAV, a Plant Virus Protein That Functions like Ribonuclease H1 and is Cytotoxic to Glioma Cells-Reference-Cited by-同舟云学术

Cauliflower Mosaic Virus TAV, a Plant Virus Protein That Functions like Ribonuclease H1 and is Cytotoxic to Glioma Cells

Published:2020-03-16 Issue: Volume:2020 Page:1-10
ISSN:2314-6133
Container-title:BioMed Research International
language:en
Short-container-title:BioMed Research International

Author:

Turri Valentina¹^ORCID,Latinovic Olga S.²³,Bonafè Massimiliano⁴,Toyang Ngeh²⁵,Parigi Maria⁶⁷,Calassanzio Matteo⁸,Martelli Pier Luigi⁹,Vagheggini Alessandro¹⁰^ORCID,Abbati Giulia¹¹,Sarnelli Anna¹²,Casadio Rita⁹,Ratti Claudio⁸,Massi Paola⁶,Schoelz James E.¹³,Salvato Maria S.²,Piccinini Filippo¹⁴,Martinelli Giovanni¹⁴

Affiliation:

1. Healthcare Direction, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, 47014 Meldola, FC, Italy

2. Institute of Human Virology (IHV), University of Maryland, School of Medicine, 21201 Baltimore, MD, USA

3. Department of Microbiology and Immunology, University of Maryland, School of Medicine, 21201 Baltimore, MD, USA

4. Department of Experimental, Diagnostic and Specialty Medicine, University of Bologna, 40126 Bologna, Italy

5. Educational and Scientific (LLC), 21201 Baltimore, MD, USA

6. Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna (IZSLER), 47122 Forlì, Italy

7. Istituto Oncologico Romagnolo (IOR), 47122 Forlì, Italy

8. Department of Agricultural and Food Sciences, School of Agriculture and Veterinary Medicine, University of Bologna, 40127 Bologna, Italy

9. Department of Pharmacy and Biotechnology, Interdepartmental Centre “L. Galvani” for Integrated Studies of Bioinformatics, Biophysics and Biocomplexity, Biocomputing Group, University of Bologna, 40126 Bologna, Italy

10. Unit of Biostatistics and Clinical Trials, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, 47014 Meldola, FC, Italy

11. Biosciences Laboratory, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, 47014 Meldola, FC, Italy

12. Medical Physics Unit, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, 47014 Meldola, FC, Italy

13. Division of Plant Sciences, University of Missouri, 65211 Columbia, MO, USA

14. Scientific Directorate, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, 47014 Meldola, FC, Italy

Abstract

Recent comparisons between plant and animal viruses reveal many common principles that underlie how all viruses express their genetic material, amplify their genomes, and link virion assembly with replication. Cauliflower mosaic virus (CaMV) is not infectious for human beings. Here, we show that CaMV transactivator/viroplasmin protein (TAV) shares sequence similarity with and behaves like the human ribonuclease H1 (RNase H1) in reducing DNA/RNA hybrids detected with S9.6 antibody in HEK293T cells. We showed that TAV is clearly expressed in the cytosol and in the nuclei of transiently transfected human cells, similar to its distribution in plants. TAV also showed remarkable cytotoxic effects in U251 human glioma cells in vitro. These characteristics pave the way for future analysis on the use of the plant virus protein TAV, as an alternative to human RNAse H1 during gene therapy in human cells.

Funder

Paul G. Allen Family Foundation

Publisher

Hindawi Limited

Subject

General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Link

http://downloads.hindawi.com/journals/bmri/2020/7465242.pdf

Reference43 articles.

1. Oncolytic myxoma virus: The path to clinic

2. Plant virus emergence and evolution: Origins, new encounter scenarios, factors driving emergence, effects of changing world conditions, and prospects for control

3. Evidence that a plant virus switched hosts to infect a vertebrate and then recombined with a vertebrate-infecting virus

4. Building bridges between plant and animal viruses

5. Pararetroviruses and Retroviruses: A Comparative Review of Viral Structure and Gene Expression Strategies

Cited by 3 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Cauliflower mosaic virus: Virus-host interactions and its uses in biotechnology and medicine;Virology;2023-03

2. Plants as Edible Vaccine;Plants as Bioreactors for Industrial Molecules;2023-02-03

3. Transcriptomics, molecular docking, and cross-resistance profiling of nobiletin in cancer cells and synergistic interaction with doxorubicin upon SOX5 transfection;Phytomedicine;2022-06