Application of Genomic In Situ Hybridization in Horticultural Science

Author:

Ramzan Fahad1,Younis Adnan2ORCID,Lim Ki-Byung13

Affiliation:

1. Department of Horticulture, Kyungpook National University, Daegu 41566, Republic of Korea

2. Institute of Horticultural Sciences, University of Agriculture, Faisalabad 38040, Pakistan

3. Institute of Agricultural Science and Technology, Kyungpook National University, Daegu 41566, Republic of Korea

Abstract

Molecular cytogenetic techniques, such as in situ hybridization methods, are admirable tools to analyze the genomic structure and function, chromosome constituents, recombination patterns, alien gene introgression, genome evolution, aneuploidy, and polyploidy and also genome constitution visualization and chromosome discrimination from different genomes in allopolyploids of various horticultural crops. Using GISH advancement as multicolor detection is a significant approach to analyze the small and numerous chromosomes in fruit species, for example,Diospyroshybrids. This analytical technique has proved to be the most exact and effective way for hybrid status confirmation and helps remarkably to distinguish donor parental genomes in hybrids such asClivia,Rhododendron, andLycorisornamental hybrids. The genome characterization facilitates in hybrid selection having potential desirable characteristics during the early hybridization breeding, as this technique expedites to detect introgressed sequence chromosomes. This review study epitomizes applications and advancements of genomic in situ hybridization (GISH) techniques in horticultural plants.

Funder

Ministry of Education

Publisher

Hindawi Limited

Subject

Pharmaceutical Science,Genetics,Molecular Biology,Biochemistry

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