Application of Biosynthesized Silver Nanoparticles for the Control of Land SnailEobania vermiculataand Some Plant Pathogenic Fungi

Abstract

The land snailEobania vermiculatais an important crop pest causing considerable damage in agriculture. The aim of the present work is to evaluate the possibilities of using silver nanoparticles (AgNPs) to control the land snail. The AgNPs have been synthesized biologically using white radish (Raphanus sativusvar.aegyptiacus). The biosynthesis was regularly monitored by UV-Vis spectroscopy. X-ray diffraction spectra revealed peaks of crystalline nature of AgNPs and the transmission electron micrographs further confirmed the size of the synthesized nanoparticles ranging from 6 to 38 nm. The exposure of the snails and soil matrix to AgNPs in a laboratory experiment reduced the activity and the viability of the land snail (20% of AgNPs treated snails died) as well as the frequency of fungal population in the surrounding soil. Moreover histology and ultrastructure alterations have been found in both kidney and the digestive gland of AgNPs treated land snails. The synergistic effect of synthesized AgNPs as antifungal was evaluated and clearly revealed that AgNPs can be effectively used against various plant pathogenic fungi. The present study results may open a new avenue to use the snail as bioindicator organism of environmental pollution.