Effect of Ocular Hypertension on D-β-Aspartic Acid-Containing Proteins in the Retinas of Rats-Reference-Cited by-同舟云学术

Effect of Ocular Hypertension on D-β-Aspartic Acid-Containing Proteins in the Retinas of Rats

Published:2019-05-21 Issue: Volume:2019 Page:1-8
ISSN:2090-004X
Container-title:Journal of Ophthalmology
language:en
Short-container-title:Journal of Ophthalmology

Author:

Kanamoto Takashi¹^ORCID,Tachibana Takashi²,Kitaoka Yasushi³^ORCID,Hisatomi Toshio²,Ikeda Yasuhiro²,Murakami Yusuke²,Tobiume Kei⁴,Asaoka Ryo⁵^ORCID,Kiuchi Yoshiaki⁶

Affiliation:

1. Department of Ophthalmology, Hiroshima Memorial Hospital, 1-4-3, Honkawacho, Naka-ku, Hiroshima 730-0802, Japan

2. Department of Ophthalmology, Graduate School of Medical Sciences, Kyushu University, 3-1-1, Maidashi, Higashi-ku, Fukuoka 812-8582, Japan

3. Department of Ophthalmology, St. Marianna University School of Medicine, 2-16-1, Sugao, Miyamae-ku, Kawasaki City, Kanagawa 216-8511, Japan

4. Graduate School of Biomedical & Health Sciences, Hiroshima University, 1-2-3, Kasumi, Minami-ku, Hiroshima 734-8551, Japan

5. Department of Ophthalmology, University of Tokyo Graduate School of Medicine, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan

6. Department of Ophthalmology and Visual Sciences, Hiroshima University, 1-2-3, Kasumi, Minami-ku, Hiroshima 734-8551, Japan

Abstract

Purpose. To investigate the effect of ocular hypertension-induced isomerization of aspartic acid in retinal proteins. Methods. Adult Wistar rats with ocular hypertension were used as an experimental model. D-β-aspartic acid-containing proteins were isolated by SDS-PAGE and western blot with an anti-D-β-aspartic acid antibody and identified by liquid chromatography-mass spectrometry analysis. The concentration of ATP was measured by ELISA. Results. D-β-aspartic acid was expressed in a protein band at around 44.5 kDa at much higher quantities in the retinas of rats with ocular hypertension than in those of normotensive rats. The 44.5 kDa protein band was mainly composed of α-enolase, S-arrestin, and ATP synthase subunits α and β, in both the ocular hypertensive and normotensive retinas. Moreover, increasing intraocular pressure was correlated with increasing ATP concentrations in the retinas of rats. Conclusion. Ocular hypertension affected the expression of proteins containing D-β-aspartic acid, including ATP synthase subunits, and up-regulation of ATP in the retinas of rats.

Publisher

Hindawi Limited

Subject

Ophthalmology

Link

http://downloads.hindawi.com/journals/joph/2019/2431481.pdf

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