High-Fat Diet Induces Oxidative Stress and MPK2 and HSP83 Gene Expression inDrosophila melanogaster

Author:

Trindade de Paula Mariane1,Poetini Silva Márcia Rósula1,Machado Araujo Stífani1,Cardoso Bortolotto Vandreza1,Barreto Meichtry Luana1,Zemolin Ana Paula Pegoraro2,Wallau Gabriel L.3,Jesse Cristiano Ricardo1,Franco Jeferson Luís3ORCID,Posser Thaís3,Prigol Marina1ORCID

Affiliation:

1. Laboratório de Avaliações Farmacológicas e Toxicológicas Aplicadas às Moléculas Bioativas, Universidade Federal do Pampa (Unipampa), Campus Itaqui, 97650-000 Itaqui, RS, Brazil

2. Programa de Pós Graduação em Ciências Biológicas: Bioquímica Toxicológica, Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, Brazil

3. Centro Interdisciplinar de Pesquisa em Biotecnologia (CIP/BIOTEC), Universidade Federal do Pampa, Campus São Gabriel, 97300 000 São Gabriel, RS, Brazil

Abstract

The consumption of a high-fat diet (HFD) causes alteration in normal metabolism affecting lifespan of flies; however molecular mechanism associated with this damage in flies is not well known. This study evaluates the effects of ingestion of a diet supplemented with 10% and 20% of coconut oil, which is rich in saturated fatty acids, on oxidative stress and cells stress signaling pathways. After exposure to the diet for seven days, cellular and mitochondrial viability, lipid peroxidation and antioxidant enzymes SOD and CAT activity, and mRNA expression of antioxidant enzymes HSP83 and MPK2 were analyzed. To confirm the damage effect of diet on flies, survival and lifespan were investigated. The results revealed that the HFD augmented the rate of lipid peroxidation and SOD and CAT activity and induced a higher expression of HSP83 and MPK2 mRNA. In parallel, levels of enzymes involved in lipid metabolism (ACSL1 and ACeCS1) were increased. Our data demonstrate that association among metabolic changes, oxidative stress, and protein signalization might be involved in shortening the lifespan of flies fed with a HFD.

Funder

Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul

Publisher

Hindawi Limited

Subject

Cell Biology,Ageing,General Medicine,Biochemistry

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