Comparative Genomics of Actinobacillus pleuropneumoniae Serotype 8 Reveals the Importance of Prophages in the Genetic Variability of the Species

Author:

Prado Isabelle Gonçalves de Oliveira1ORCID,da Silva Giarlã Cunha1,Crispim Josicelli Souza1,Vidigal Pedro Marcus Pereira2ORCID,Nascimento Moysés3,Santana Mateus Ferreira1,Bazzolli Denise Mara Soares1ORCID

Affiliation:

1. Laboratório de Genética Molecular de Bactérias/Bioagro-Departamento de Microbiologia, Universidade Federal de Viçosa, Viçosa 36570-900, Brazil

2. Núcleo de Análise de Biomoléculas (NuBioMol), Universidade Federal de Viçosa, Viçosa, Brazil

3. Departamento de Estatística, Universidade Federal de Viçosa, Viçosa, Brazil

Abstract

Actinobacillus pleuropneumoniae is the etiologic agent of porcine pleuropneumonia. Currently, there are 18 different serotypes; the serotype 8 is the most widely distributed in the United States, Canada, United Kingdom, and southeastern Brazil. In this study, genomes of seven A. pleuropneumoniae serotype 8 clinical isolates were compared to the other genomes of twelve serotypes. The analyses of serotype 8 genomes resulted in a set of 2352 protein-coding sequences. Of these sequences, 76.6% are present in all serotypes, 18.5% are shared with some serotypes, and 4.9% were differential. This differential portion was characterized as a series of hypothetical and regulatory protein sequences: mobile element sequence. Synteny analysis demonstrated possible events of gene recombination and acquisition by horizontal gene transfer (HGT) in this species. A total of 30 sequences related to prophages were identified in the genomes. These sequences represented 0.3 to 3.5% of the genome of the strains analyzed, and 16 of them contained complete prophages. Similarity analysis between complete prophage sequences evidenced a possible HGT with species belonging to the family Pasteurellaceae. Thus, mobile genetic elements, such as prophages, are important components of the differential portion of the A. pleuropneumoniae genome and demonstrate a central role in the evolution of the species. This study represents the first study done to understand the genome of A. pleuropneumoniae serotype 8.

Funder

Conselho Nacional de Desenvolvimento Científico e Tecnológico

Publisher

Hindawi Limited

Subject

Pharmaceutical Science,Genetics,Molecular Biology,Biochemistry

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