Genetic Diversity Assessment of MPOB-Senegal Oil Palm Germplasm Using Microsatellite Markers

Author:

Myint Khin Aye1,Yaakub Zulkifli2ORCID,Rafii Mohd Y.1ORCID,Oladosu Yusuff1,Samad Mohd Yusoff Abd3,Ramlee Shairul Izan4,Mustaffa Suzana2,Arolu Fatai1,Abdullah Norziha2,Marjuni Marhalil2,Amiruddin Mohd Din2

Affiliation:

1. Laboratory of Climate-Smart Food Crop Production, Institute of Tropical Agriculture and Food Security, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia

2. Advanced Biotechnology and Breeding Centre, Malaysian Palm Oil Board, 6, Persiaran Institusi, Bandar Baru Bangi, 43000 Kajang, Selangor, Malaysia

3. Department of Soil Management, Faculty of Agriculture, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia

4. Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia

Abstract

Molecular characterization of oil palm germplasm is crucial in utilizing and conserving germplasm with promising traits. This study was conducted to evaluate the genetic diversity structures and relationships among 26 families of MPOB-Senegal oil palm germplasm using thirty-five microsatellite markers. High level of polymorphism ( P = 96.26 %), number of effective allele ( N e = 2.653 ), observed heterozygosity ( H o = 0.584 ), expected heterozygosity ( H e = 0.550 ), total heterozygosity ( H T = 0.666 ), and rare alleles (54) were observed which indicates that MPOB-Senegal germplasm has a broad genetic variation. Among the SSR markers, sMo00053 and sMg00133 were the most informative markers for discrimination among the MPOB-Senegal oil palm germplasm for having the highest private alleles and the rare alleles. For selection and conservation, oil palm populations with high rare alleles and Nei’s gene diversity index should be considered as these populations may possess unique genes for further exploitation.

Funder

Universiti Putra Malaysia

Publisher

Hindawi Limited

Subject

General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

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