Monitoring EDTA and endogenous metabolite biomarkers from serum with mass spectrometry

Author:

Lowe Rachel12,Go Eden P.1,Tong Grace C.1,Voelcker Nicolas H.2,Siuzdak Gary1

Affiliation:

1. Department of Molecular Biology, The Scripps Center of Mass Spectrometry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA

2. School of Chemistry Physics and Earth Sciences, Flinders University, Bedford Park, 5042, South Australia, Australia

Abstract

We describe a quantitative method for the determination of ethylenediaminetetraacetic acid (EDTA) in human serum by gas chromatography mass spectrometry (GC-MS), liquid chromatography electrospray ionisation tandem mass spectrometry (LC-ESI-MS/MS), and desorption ionisation on silicon mass spectrometry (DIOS-MS). In the initial stages of the analysis, endogenous metabolites (1-palmitoyl-sn-glycero-3-phosphocholine and 1-stearoyl-sn-glycero-3-phosphocholine) were readily observed in LC-ESI-MS and DIOS-MS however, direct analysis of the EDTA free acid had limited sensitivity. In order to improve EDTA detection we employed a straightforward esterification derivatization. The most successful derivatization procedure converted EDTA to its methyl ester and, since13C isotopes of these reagents are readily available, internal standards could be easily generated for quantitative analysis. This approach provided a limit of detection of 0.5 and 0.1 μM for GC-MS and LC-ESI-MS, and offers a viable method for the EDTA detection.

Funder

Mass Consortium Corporation

Publisher

Hindawi Limited

Subject

Spectroscopy

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