Affiliation:
1. Medical College of Henan University, Kaifeng 475004, China
2. Medical College, Henan Polytechnic University, Jiaozuo 454000, China
3. The First Affiliated Hospital of Henan University, Kaifeng 475001, China
Abstract
Somatostatin (SST), an endogenous peptide, may exert anti-inflammatory and neuroprotective effects on retinal injury induced by ischemia. Retinal ischemic reperfusion (I/R) injury always produces many reactive oxygen species (ROS), which can aggravate the tissue damage. The effects of octreotide (OCT), a SST analogue, on retinal I/R injury and ROS formation, are not very clear. In this study, we observed the effects of OCT on morphological changes, oxidative stress, and cell death, induced by retinal I/R injury. The activation of nuclear factorκB (NF-κB) and intercellular adhesion molecule-1 (ICAM-1) were further evaluated in I/R retina treated with or without OCT. The retinal layer thickness was increased at 1 day after I/R and decreased at 7 days after I/RP<0.05. This effect was associated with increase in MDA and ROS levelsP<0.05. The Tunel-positive cells increased and the number of ganglion cell layer (GCL) neurons decreased significantly after I/R injury. The expression of p-p65 and ICAM-1 increased significantly in I/R retinasP<0.05. Each effect was markedly attenuated by application of OCT. These data indicate that OCT protects the retina against retinal I/R damage, which could be through inhibition of oxidative stress and downregulation of NF-κB and ICAM-1 expression.
Funder
National Natural Science Foundation of China
Subject
Cell Biology,Aging,General Medicine,Biochemistry
Cited by
30 articles.
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