Ferromagnetic Levan Composite: An Affinity Matrix to Purify Lectin-Reference-Cited by-同舟云学术

Ferromagnetic Levan Composite: An Affinity Matrix to Purify Lectin

Published:2009 Issue: Volume:2009 Page:1-6
ISSN:1110-7243
Container-title:Journal of Biomedicine and Biotechnology
language:en
Short-container-title:Journal of Biomedicine and Biotechnology

Author:

Angeli Renata¹²,da Paz Nathalia V. N.¹,Maciel Jackeline C.¹³,Araújo Flávia F. B.¹,Paiva Patrícia M. G.¹,Calazans Glícia M. T.⁴,Valente Ana Paula²,Almeida Fábio C. L.²,Coelho Luana C. B. B.¹,Carvalho Luiz B.¹²,Silva Maria da Paz C.¹²,Correia Maria Tereza dos Santos¹

Affiliation:

1. Laboratório de Glicoproteínas, Departamento de Bioquímica, Universidade Federal de Pernambuco (UFPE), 50670-901 Recife, PE, Brazil

2. Centro Nacional de Ressonância Magnética Nuclear de Macromoléculas, Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro, 21941-590 Rio de Janeiro, RJ, Brazil

3. Laboratório de Imunopatologia Keizo Asami (LIKA), UFPE, 50670-901 Recife, PE, Brazil

4. Departamento de Antibióticos, Universidade Federal de Pernambuco (UFPE), 50670-901 Recife, PE, Brazil

Abstract

A simple and inexpensive procedure used magnetite and levan to synthesize a composite recovered by a magnetic field. Lectins fromCanavalia ensiformis(Con A) andCratylia mollis(Cramoll 1 and Cramoll 1,4) did bind specifically to composite. The magnetic property of derivative favored washing out contaminating proteins and recovery of pure lectins with glucose elution. Cramoll 1 was purified by this affinity binding procedure in two steps instead of a previous three-step protocol with ammonium sulfate fractionation, affinity chromatography on Sephadex G-75, and ion exchange chromatography through a CM-cellulose column.

Funder

Conselho Nacional de Desenvolvimento Científico e Tecnológico

Publisher

Hindawi Limited

Subject

Health, Toxicology and Mutagenesis,Genetics,Molecular Biology,Molecular Medicine,General Medicine,Biotechnology

Link

http://downloads.hindawi.com/journals/bmri/2009/179106.pdf

Reference30 articles.

1. Solid-phase chemical tools for glycobiology

2. Immobilization of protein on ferromagnetic Dacron

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