Protection against Amoebic Liver Abscess in Hamster by Intramuscular Immunization with anAutographa californicaBaculovirus Driving the Expression of the Gal-Lectin LC3 Fragment

Author:

Meneses-Ruiz Dulce María1,Aguilar-Diaz Hugo1,Bobes Raúl José1,Sampieri Alicia2,Vaca Luis2ORCID,Laclette Juan Pedro1,Carrero Julio César1

Affiliation:

1. Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad Universitaria, 04510 México, DF, Mexico

2. Departamento de Biología Celular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Ciudad Universitaria, 04510 México, DF, Mexico

Abstract

In a previous study, we demonstrated that oral immunization usingAutographa californicabaculovirus driving the expression of the Gal-lectin LC3 fragment (AcNPV-LC3) ofEntamoeba histolyticaconferred protection against ALA development in hamsters. In this study, we determined the ability of AcNPV-LC3 to protect against ALA by the intramuscular route as well as the liver immune response associated with protection. Results showed that 55% of hamsters IM immunized with AcNPV-LC3 showed sterile protection against ALA, whereas other 20% showed reduction in the size and extent of abscesses, resulting in some protection in 75% of animals compared to the sham control group. Levels of protection showed a linear correlation with the development and intensity of specific antiamoeba cellular and humoral responses, evaluated in serum and spleen of hamsters, respectively. Evaluation of the Th1/Th2 cytokine patterns expressed in the liver of hamsters showed that sterile protection was associated with the production of high levels of IFNγand IL-4. These results suggest that the baculovirus system is equally efficient by the intramuscular as well as the oral routes for ALA protection and that the Gal-lectin LC3 fragment is a highly protective antigen against hepatic amoebiasis through the local induction of IFNγand IL-4.

Funder

Consejo Nacional de Ciencia y Tecnología

Publisher

Hindawi Limited

Subject

General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

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