Antihyaluronidase and Alkaline Phosphatase (ALP) Activities of Medicinal Plants to Combat Echis carinatus Venom-Induced Toxicities

Author:

Fatima Syeda1,Aslam Nazia1,Khalid Sofia1,Ullah Kalim2,Abbas Khizar3,Hussain Shahzad4,Shah Syed Sajid Hussain5,Qureshi Zia-Ur-Rahman6,Qayum Mughal7,Asad Muhammad Hassham Hassan Bin8ORCID

Affiliation:

1. Department of Environmental Sciences, Fatima Jinnah Women University, Rawalpindi, Pakistan

2. Department of Zoology, Kohat University of Science and Technology (KUST), Kohat, Pakistan

3. Department of Pharmacognosy, Faculty of Pharmacy, B.Z. University, Multan, Pakistan

4. Drugs Control & Traditional Medicines Division, National Institute of Health, Islamabad, Pakistan

5. Department of Environmental Sciences, COMSATS University Islamabad, Abbottabad Campus, 22060 KPK, Pakistan

6. Department of Pharmacy, SBK Women University, Quetta, Baluchistan, Pakistan

7. Department of Pharmacy, Kohat University of Science and Technology (KUST), Kohat, Pakistan

8. Department of Pharmacy, COMSATS University Islamabad, Abbottabad Campus, 22060 KPK, Pakistan

Abstract

Snakebite is one of the most neglected diseases of developing countries. Deaths due to snakebite envenoming are quite high in Pakistan, and many deaths are caused by Echis carinatus envenomation. Traditional use of medicinal plants against snakebites is a common practice in Pakistan due to countless benefits. The current study was performed with the objective to evaluate eighteen Pakistani medicinal plants inhibitory potential against hyaluronidase and alkaline phosphatase enzymes of Pakistani Echis carinatus venom. Hyaluronidase activity (0.2-1.6 mg/0.1 mL) and alkaline phosphatase activity (0.1-0.8 mg/0.1 mL) were measured in dose-dependent manner. Crude methanolic extracts of medicinal plants were used for in vitro investigation of their inhibitory activity against toxic enzymes. All active plants were fractioned using different solvents and were again analyzed for inhibitory activity of same enzymes. Results indicated all plants were able to neutralize hyaluronidase that Swertia chirayita (Roxb. ex Flem.) Karst., Terminalia arjuna Wight and Arn, Rubia cordifolia Thumb., and Matthiola incana (L.) R.Br. inhibited maximum hyaluronidase activity equivalent to standard reference ( p > 0.5 ). Pakistani medicinal plants are dense with natural neutralizing metabolites and other active phytochemicals which could inhibit hyaluronidase activity of Pakistani Echis carinatus venom. Further advanced studies at molecular level could lead us to an alternative for envenoming of Pakistani Echis carinatus venom.

Publisher

Hindawi Limited

Subject

General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Reference53 articles.

1. Assessment of human-snake interaction and its outcomes in Monduli District, northern Tanzania;H. E. Nonga;Tanzania Journal of Health Research,2015

2. Vulnerability to snakebite envenoming: a global mapping of hotspots

3. Snake Envenoming: A Disease of Poverty

4. New approaches and technologies of venomics to meet the challenge of human envenoming by snakebites in India;D. A. Warrell;Indian Journal of Medical Research,2013

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