To Investigate Whether Hematocrit Affects Thromboelastography Parameters

Abstract

Background. Thromboelastogram (TEG) is an experiment to detect coagulation function with whole blood. Red blood cell (RBC) is the most abundant component of blood. Whether RBC has an impact on the results of thromboelastogram? Study Design and Methods. The correlation between hematocrit (HCT) and TEG was analyzed. 17 samples were reconstituted with different HCT. They were tested separately. Correction tests were performed on 17 samples from patients with anemia. HCT was corrected to 0.40 in female and 0.45 in males. The correction formula was determined according to the experimental correction. Results. HCT was negatively correlated with TEG parameters. As HCT increased, CI and angle decreased ( $P<0.05$ , $P<0.001$ ) and K increased ( $P<0.001$ ) in reconstituted samples. In the correction test, the angle measured value was 69.48 ± 4.98 and corrected value was 62.48 ± 6.25, MA measured value was 61.44 ± 7.10 and corrected value was 55.94 ± 7.12, K measured value was 1.45 ± 0.48 and corrected value was 2.11 ± 0.79, and CI measured value was 1.07 ± 1.67 and corrected value was −0.45 ± 1.64. There was a significant difference. The correction formulas of anemia were derived from the experimental correction results. KCorrection value = (0.7903∗A2 − 2.1803A + 2.8268)∗KMeasured value; Tan angleCorrection value = Tan angleMeasured value/(0.6596∗A2 − 1.7478A + 2.4608); MACorrection value = MAMeasured value/(0.1853ln (A) + 1.0197); CICorrection value = −0.6516RMeasured value − 0.3772KCorrection value + 0.1224MACorrection value + 0.0759angleCorrection value − 7.7922. Conclusion. HCT has a negative impact on TEG parameters. Coagulation state of anemia patients is overestimated by TEG. The test results of anemia patients need to be corrected whether through the experimental correction or the formula correction.