Protective Effect of Galectin-1 duringHistoplasma capsulatumInfection Is Associated with Prostaglandin E2and Nitric Oxide Modulation

Abstract

Histoplasma capsulatumis a dimorphic fungus that develops a yeast-like morphology in host’s tissue, responsible for the pulmonary disease histoplasmosis. The recent increase in the incidence of histoplasmosis in immunocompromised patients highlights the need of understanding immunological controls of fungal infections. Here, we describe our discovery of the role of endogenous galectin-1 (Gal-1) in the immune pathophysiology of experimental histoplasmosis. All infected wild-type (WT) mice survived while only 1/3 of Lgals1−/−mice genetically deficient in Gal-1 survived 30 days after infection. Although infected Lgals1−/−mice had increased proinflammatory cytokines, nitric oxide (NO), and elevations in neutrophil pulmonary infiltration, they presented higher fungal load in lungs and spleen. Infected lung and infected macrophages from Lgals1−/−mice exhibited elevated levels of prostaglandin E2(PGE2, a prostanoid regulator of macrophage activation) and prostaglandin E synthase 2 (Ptgs2) mRNA. Gal-1 did not bind to cell surface of yeast phase ofH. capsulatum,in vitro, suggesting that Gal-1 contributed to phagocytes response to infection rather than directly killing the yeast.The data provides the first demonstration of endogenous Gal-1 in the protective immune response againstH. capsulatumassociated with NO and PGE2as an important lipid mediator in the pathogenesis of histoplasmosis.