Affiliation:
1. Department of General Practice, Shenzhen Luohu People’s Hospital, The 3rd Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong, China
2. Department of Health Care, The Shunde Affiliated Hospital of Southern Medical University, Guangzhou, Guangdong, China
3. Department of Endocrinology and Metabolism, Shenzhen Luohu People’s Hospital, The 3rd Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong, China
Abstract
Objective. Circulating miRNAs are acclaimed biomarkers to predict the occurrence and progression of type 2 diabetes mellitus (T2DM). This study is aimed at analyzing the correlation of circulating miR-210 level and obesity-associated T2DM and then investigating the underlying mechanism of circulating miR-210 in T2DM. Methods. Totally, 137 serum samples from patients with T2DM were collected; meanwhile, the demographic, general, and clinical hematological characteristics, disease history, and dietary patterns were recorded. The miR-210 level in exosomes from serum was detected by qRT-PCR. Then, the correlations of BMI or miR-210 level with patients’ clinical characteristics were analyzed. Furthermore, the miR-210 level was detected in T2DM related various cells under high glucose condition. Meanwhile, the expression of carbohydrate responsive element binding protein (ChREBP) and hypoxia-inducible factor 1α (HIF-1α) was measured by western blotting. Results. The miR-210 level in exosomes from serum was obviously elevated in the
group compared with the
group. Higher BMI was correlated with abnormal lipid metabolism and impaired liver function as well as higher miR-210 level. Notably, higher miR-210 level was also correlated with abnormal lipid metabolism, disease history, and dietary patterns. In addition, compared with normal cells, high glucose increased the miR-210 level in exosomes from cell culture supernatants as well as cells in HUVEC, VSMC, RAW 264.7, 3 T3-L1, SMC, and Beta-TC-6 cells, while it reduced the expression of ChREBP and HIF-1α. Conclusions. Circulating miR-210 level was closely correlated with obesity-associated T2DM. Furthermore, higher miR-210 level might be implicated in the occurrence and development of T2DM and its complications.
Funder
Shenzhen Key Medical Discipline Construction
Subject
Endocrinology,Endocrinology, Diabetes and Metabolism
Cited by
4 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献