Metal-Based Nanoparticles Enhance Drought Tolerance in Soybean

Abstract

Drought is a major abiotic stress that negatively impacts plant growth and crop production. Among various techniques used to alleviate drought stress in plants, nanoparticle application is considered to be effective and promising. In this study, the responses of plants treated with iron, copper, cobalt, and zinc oxide nanoparticles (NPs) were analyzed in soybean under drought-induced conditions. The obtained results indicated that these metal-based NPs supported the drought tolerance of NP-treated plants. The desired physiological traits, viz., relative water content, drought tolerance index, and biomass reduction rate, were significantly improved, especially in iron NP-treated plants. At the molecular level, quantitative PCR analysis of several drought-responsive genes revealed a gene-, tissue-, and NP-dependent upregulation of gene expression. Iron NP treatment promoted the expression of all tested genes in roots; additionally, the expression of three drought-responsive genes increased in leaves of all NP-treated plants, while the expression of GmERD1 (Early Responsive to Dehydration 1) was induced in both roots and shoots under the four NP treatments tested. Our findings suggest that NP application can improve drought tolerance of soybean plants by triggering drought-associated gene expression.