Common Variable Immunodeficiency and Circulating TFH

Abstract

CD4+ T follicular helper cells (TFH) were assessed in adult patients with common variable immune deficiency (CVID) classified according to the presence of granulomatous disease (GD), autoimmunity (AI), or both GD and AI (Group I) or the absence of AI and GD (Group II).TFHlymphocytes were characterized by expression of CXCR5 and PD-1.TFHwere higher (in both absolute number and percentage) in Group I than in Group II CVID patients and normal controls (N). Within CXCR5+CD4+ T cells, the percentage of PD-1 (+) was higher and that of CCR7 (+) was lower in Group I than in Group II and N. The percentages of Treg andTFHreg were similar in both CVID groups and in N.TFHresponded to stimulation increasing the expression of the costimulatory molecules CD40L and ICOS as did N. After submitogenic PHA+IL-2 stimulation, intracellular expression ofTFHcytokines (IL-10, IL-21) was higher than N in Group I, and IL-4 was higher than N in Group II. These results suggest thatTFHare functional in CVID and highlight the association of increased circulatingTFHwith AI and GD manifestations.