Increased Resistance of Breast, Prostate, and Embryonic Carcinoma Cells against Herpes Simplex Virus in Three-Dimensional Cultures

Author:

Voros Andras1,Kormos Bernadett1ORCID,Valyi-Nagy Tibor1ORCID,Valyi-Nagy Klara1ORCID

Affiliation:

1. Department of Pathology, College of Medicine, University of Illinois at Chicago, Chicago, IL 60612, USA

Abstract

In previous studies we found that uveal melanoma cells grown in extracellular matrix (ECM)-containing three-dimensional (3D) cultures have increased resistance against herpes simplex virus type 1 (HSV-1)-mediated destruction relative to cells cultured without ECM. Using additional tumor cell types including MB-231 human breast cancer cells, PC-3 human prostate cancer cells, and P19 mouse embryonal carcinoma cells, we show here that tumor cell lines other than melanoma are also more resistant to HSV-1-mediated destruction in 3D cultures than cells grown in 2D. We also demonstrate here that one mechanism responsible for the increased resistance of tumor cells to HSV-1 infection in 3D cultures is an ECM-mediated inhibition of virus replication following virus entry into cells. These findings confirm and extend previous observations related to the role of the ECM in tumor resistance against HSV-1 and may lead to improved strategies of oncolytic virotherapy.

Publisher

Hindawi Limited

Subject

Pulmonary and Respiratory Medicine,Pediatrics, Perinatology, and Child Health

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