Detection ofTrichomonas vaginalisUsing the Polymerase Chain Reaction in Pregnant and Non-Pregnant Women

Author:

Jeremias J.1,Draper D.2,Ziegert M.1,Jones W.2,Inglis S.1,McGregor J. A.2,Witkin S. S.1

Affiliation:

1. Department of Obstetrics and Gynecology, Cornell University Medical College, 515 East 71st Street, New York, NY 10021, USA

2. Department of Obstetrics and Gynecology, University of Colorado Health Science Center, Denver, CO, USA

Abstract

Objective:Trichomonas vaginalisvaginal infections are often both asymptomatic and difficult to detect by current methods. We evaluated the ability of a newly developed polymerase chain reaction (PCR) assay to identifyT. vaginalisin vaginal samples from pregnant and non-pregnant women.Methods:In the 1st study, we compared the prevalence ofT. vaginalisdetection by PCR and culture using Diamond's medium in 52 women with symptoms of vaginal infection. In the 2nd study,T. vaginaliswas detected using PCR and wet mount microscopy in 131 asymptomatic pregnant women.Results:Among the women with symptoms of vaginitis, 7 (13.5%) were PCR-positive forT. vaginalis. Six of the PCR-positive women, but none of the PCR-negative women, were culture-positive for this organism. All but 1 of the women with candidal vaginitis or bacterial vaginosis were PCR-negative for T.vaginalis. Among the asymptomatic pregnant women, all of whom were negative forT. vaginalisby wet mount, l0 (7.6%) were PCR-positive forT. vaginalis.Conclusions:PCR offers a rapid and sensitive alternative to culture and microscopy for the detection ofT. vaginalisvaginal infections in both symptomatic and asymptomatic women.

Funder

Eleanor Naylor Dana Charitable Trust

Publisher

Hindawi Limited

Subject

Infectious Diseases,Obstetrics and Gynaecology,Dermatology

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