Evaluation ofhsp65Nested PCR-Restriction Analysis (PRA) for Diagnosing Tuberculosis in a High Burden Country-Reference-Cited by-同舟云学术

Evaluation ofhsp65Nested PCR-Restriction Analysis (PRA) for Diagnosing Tuberculosis in a High Burden Country

Published:2013 Issue: Volume:2013 Page:1-6
ISSN:2314-6133
Container-title:BioMed Research International
language:en
Short-container-title:BioMed Research International

Author:

Macente Sara¹,Fujimura Leite Clarice Queico²,Santos Adolfo Carlos Barreto²,Siqueira Vera Lúcia Dias³,Machado Luzia Neri Cosmo⁴,Marcondes Nadir Rodrigues⁴,Hirata Mario Hiroyuki⁵,Hirata Rosário Dominguez Crespo⁵,Cardoso Rosilene Fressatti³^ORCID

Affiliation:

1. Post Graduation Program in Biosciences Applied to Pharmacy, Department of Clinical Analyses and Biomedicine, State University of Maringa, 87020-900 Maringá, PR, Brazil

2. School of Pharmaceutical Sciences, Department of Biological Sciences, Paulista State University, Araraquara, 14800-901 São Paulo, SP, Brazil

3. Department of Clinical Analyses and Biomedicine, State University of Maringa, 87020-900 Maringá, PR, Brazil

4. Center of Medical and Pharmaceutical Sciences, State University of Western of Parana, 85819-110 Cascavel, PR, Brazil

5. School of Pharmaceutical Science, University of São Paulo, 05508-900 São Paulo, SP, Brazil

Abstract

Current study evaluated thehsp65Nested PCR Restriction Fragment Length Polymorphism Analysis (hsp65Nested PCR-PRA) to detect and identifyMycobacterium tuberculosiscomplex directly in clinical samples for a rapid and specific diagnosis of tuberculosis (TB).hsp65Nested PCR-PRA was applied directly to 218 clinical samples obtained from 127 patients suspected of TB or another mycobacterial infection from July 2009 to July 2010. Thehsp65Nested PCR-PRA showed 100% sensitivity and 95.0 and 93.1% specificity in comparison with culture and microscopy (acid fast bacillus smear), respectively.hsp65Nested PCR-PRA was shown to be a fast and reliable assay for diagnosing TB, which may contribute towards a fast diagnosis that could help the selection of appropriate chemotherapeutic and early epidemiological management of the cases which are of paramount importance in a high TB burden country.

Publisher

Hindawi Limited

Subject

General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Link

http://downloads.hindawi.com/journals/bmri/2013/391549.pdf

Reference23 articles.

1. hsp65 PCR-restriction analysis (PRA) with capillary electrophoresis in comparison to three other methods for identification of Mycobacterium species

2. Rapid Identification of Mycobacteria from Smear-Positive Sputum Samples by Nested PCR-Restriction Fragment Length Polymorphism Analysis

3. An Official ATS/IDSA Statement: Diagnosis, Treatment, and Prevention of Nontuberculous Mycobacterial Diseases

4. Identification of Mycobacterium spp. by Using a Commercial 16S Ribosomal DNA Sequencing Kit and Additional Sequencing Libraries

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