Low-Level Detection of Poly(amidoamine) PAMAM Dendrimers Using Immunoimaging Scanning Probe Microscopy

Author:

Cason Chevelle A.1,Fabré Thomas A.1,Buhrlage Andrew1,Haik Kristi L.2,Bullen Heather A.1

Affiliation:

1. Department of Chemistry, Northern Kentucky University, Highland Heights, KY 41099, USA

2. Department of Biological Sciences, Northern Kentucky University, Highland Heights, KY 41099, USA

Abstract

Immunoimaging scanning probe microscopy was utilized for the low-level detection and quantification of biotinylated G4 poly(amidoamine) PAMAM dendrimers. Results were compared to those of high-performance liquid chromatography (HPLC) and found to provide a vastly improved analytical method for the low-level detection of dendrimers, improving the limit of detection by a factor of 1000 (LOD=2.5×1013 moles). The biorecognition method is reproducible and shows high specificity and good accuracy. In addition, the capture assay platform shows a promising approach to patterning dendrimers for nanotechnology applications.

Funder

Merck Institute for Science Education

Publisher

Hindawi Limited

Subject

Analytical Chemistry

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