Evaluation of Dietary Essential Amino Acid Supplementation on Growth, Digestive Capacity, Antioxidant, and Intestine Health of the Juvenile Redclaw Crayfish, Cherax quadricarinatus

Author:

Jiang Zongzheng1,Qian Dunwei12,Liang Zhenye1,Wu Sen1,Han Fenglu1ORCID,Xu Chang1ORCID,Chi Meili3ORCID,Li Erchao4ORCID

Affiliation:

1. Key Laboratory of Tropical Hydrobiology and Biotechnology of Hainan Province, Hainan Aquaculture Breeding Engineering Research Center, School of Marine Biology and Aquaculture, Hainan University, Haikou, Hainan 570228, China

2. Hainan Provincial Ecological and Environmental Monitoring Centre, 98 Baiju Road, Haikou, Hainan 571126, China

3. Key Laboratory of Healthy Freshwater Aquaculture, Ministry of Agriculture and Rural Affairs, Key Laboratory of Freshwater Aquaculture Genetic and Breeding of Zhejiang Province, Zhejiang Institute of Freshwater Fisheries, Huzhou, Zhejiang 313001, China

4. Laboratory of Aquaculture Nutrition and Environmental Health, School of Life Science, East China Normal University, 500 Dongchuan Road, Shanghai 200241, China

Abstract

The present study was an 8-week feeding trial investigating the effects of lysine and threonine supplementation in vegetable-based diets on growth, antioxidative capacity, and gut microbiota of juvenile redclaw crayfish, Cherax quadricarinatus (initial weight 11.52 ± 0.23 g). The lysine and threonine were supplemented to formulate five isonitrogenous (37%) and isolipidic (9%) diets containing 0% (control), 0.2% lysine (L0.2), 0.2% threonine (T0.2), 0.4% lysine (L0.4), and 0.4% threonine (T0.4), respectively. Compared to the control, weight gain rate (WGR) and specific growth rate (SGR) of C. quadricarinatus significantly increased with increasing dietary lysine and threonine supplementation from 0.2% to 0.4% (P<0.05). Hepatopancreas trypsin activity significantly increased with increasing levels of lysine and threonine in diets (P<0.05). However, the pepsin, lipase, and amylase activities were not affected by dietary levels of lysine and threonine (P>0.05). Compared with the control, crayfish in T0.4 and L0.4 showed significantly higher glutathione peroxidase (GPx) activity (P<0.05), lower alanine aminotransferase (ALT) activity, and lower malondialdehyde (MDA) content (P<0.05). Supplementation with 0.4% lysine significantly changed the composition of the gut microbiota (P<0.05), which showed a significantly increased relative abundance of Proteobacteria and decreased Firmicutes, Actinomycetes, and Pontomyces (P<0.05). The PICRUSt analysis demonstrated that the abundance of the metabolism and cellular processes pathways in the L0.4 group were markedly decreased compared with the control (P<0.05). Meanwhile, a tighter interaction of the microbiota community in crayfish was observed in the T0.4 experimental group. In conclusion, these results suggested that dietary supplementation with 0.4% threonine could significantly promote growth and improve microbial health in juvenile C. quadricarinatus.

Funder

Ministry of Agriculture of the People's Republic of China

Publisher

Hindawi Limited

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