Alkaloids inErythrinaby UPLC-ESI-MS andIn VivoHypotensive Potential of Extractive Preparations

Author:

Merlugo Liara12,Santos Marí C.2,Sant’Anna Liane S.13,Cordeiro Everson W. F.2,Batista Luiz A. C.2,Miotto Silvia T. S.4,Garcia Cássia V.5,Moreira Cleci M.13,Mendez Andreas S. L.15

Affiliation:

1. Programa de Pós-graduação em Bioquímica, Universidade Federal do Pampa, BR 472 Km 585, Caixa Postal 118, 97508-000 Uruguaiana, RS, Brazil

2. Laboratório de Desenvolvimento e Controle de Qualidade de Medicamentos, Universidade Federal do Pampa, BR 472 Km 585, Caixa Postal 118, 97508-000 Uruguaiana, RS, Brazil

3. Laboratório de Fisiologia Cardiovascular, Universidade Federal do Pampa, BR 472 Km 585, Caixa Postal 118, 97508-000 Uruguaiana, RS, Brazil

4. Departamento de Botânica, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil

5. Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Avenida Ipiranga 2752, 90610-000 Porto Alegre, RS, Brazil

Abstract

Erythrinaspecies are used in popular medicine as sedative, anxiolytic, anti-inflammatory, and antihypertensive. In this work, we investigated the chemical composition of extracts obtained from leaves ofE. falcataandE. crista-galli. The hypotensive potential ofE. falcataand the mechanism of action were also studied. The extracts were obtained by maceration and infusion. The total content of phenolic compounds and flavonoids was estimated by spectrophotometric methods. The chemical constituents were studied performing a chromatographic analysis by UPLC-ESI-MS. Forin vivoprotocols, blood pressure and heart rate were measured by the invasive hemodynamic monitoring method. Different concentrations of extracts and drugs such as L-NAME, losartan, hexamethonium, and propranolol were administrated i.v. The results of total phenolic contents forE. falcataandE. crista-galliwere 1.3193–1.4989 mgGAE/mL for maceration and 0.8771–0.9506 mgGAE/mL for infusion. In total flavonoids, the content was 7.7829–8.1976 mg RE/g for maceration and 9.3471–10.4765 RE mg/g for infusion. The chemical composition was based on alkaloids, suggesting the presence of erythristemine, 11β-methoxyglucoerysodine, erysothiopine, 11β-hydroxyerysodine-glucose, and 11-hydroxyerysotinone-rhamnoside. A potent dose-dependent hypotensive effect was observed forE. falcata, which may be related to the route ofβ-adrenergic receptors.

Funder

Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul

Publisher

Hindawi Limited

Subject

Complementary and alternative medicine

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