The Effect and Mechanism of Emodin on the NO Secretion of Human Umbilical Vein Endothelial Cells (HUVECs) Induced by High Glucose-Reference-Cited by-同舟云学术

The Effect and Mechanism of Emodin on the NO Secretion of Human Umbilical Vein Endothelial Cells (HUVECs) Induced by High Glucose

Published:2020-06-12 Issue: Volume:2020 Page:1-5
ISSN:1687-8337
Container-title:International Journal of Endocrinology
language:en
Short-container-title:International Journal of Endocrinology

Author:

Li Yajia¹²³,Li Qiangxiang⁴⁵⁶^ORCID,Xie Chunyan⁶,Huang Yanfei⁵,Jia Limin⁵

Affiliation:

1. Department of Dermatology, Xiangya Hospital, Central South University, Changsha, China

2. Hunan Engineering Research Center of Skin Health and Disease, Central South University, Changsha, China

3. Hunan Key Laboratory of Skin Cancer and Psoriasis, Central South University, Changsha, Hunan, China

4. National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China

5. National Clinical Research Center for Geriatric Disorders (XIANGYA), Changsha, Hunan 410008, China

6. The Loudi Affiliated Hospital, Nanhua University, Loudi, Hunan Province 417000, China

Abstract

Objective. To investigate the effects of emodin on nitric oxide (NO) secretion induced by high glucose in human umbilical vein endothelial cells (HUVECs) through the p-Akt signaling pathway. Methods. Sensitivity of cells to emodin was determined by MTT assay to establish the experimental concentrations; then, HUVECs were treated with high-dose (33.3 mmol/L) glucose (HG), HG + emodin (HG + E), HG + the Akt phosphorylation inhibitor LY294002 (HG + LY), or HG + E + LY. The p-Akt (Ser 473) expression in 48 h was analyzed using Western blot. NO effect on the secretion of HUVECs was analyzed using nitrate reductase assay. Results. The sensitive emodin concentration for HUVECs growth was 10 mol/L (P<0.05). Compared with the HG group, NO secretion was significantly higher in the HG + E group (P<0.05), whereas it was lowest in the HG + LY group (P<0.05). Compared with the HG + LY group, NO secretion was increased in the HG + E + LY group (P<0.05). The p-Akt protein expression was decreased in the HG + LY group when compared to the HG group (P<0.05), while it significantly increased in the HG + E group (P<0.05). Compared with HG + LY group, p-Akt protein expression was significantly higher in the HG + E + LY group (P<0.05). Conclusion. Emodin could improve the NO secretion of HUVECs by high glucose through the p-Akt signaling pathway.

Funder

2017 Hunan Province Technology Innovation Guidance Program Clinical Medical Technology Innovation Guidance Project

Publisher

Hindawi Limited

Subject

Endocrine and Autonomic Systems,Endocrinology,Endocrinology, Diabetes and Metabolism

Link

http://downloads.hindawi.com/journals/ije/2020/4832840.pdf

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