High Specificity of Quantitative Methylation-Specific PCR Analysis forMGMTPromoter Hypermethylation Detection in Gliomas-Reference-Cited by-同舟云学术

High Specificity of Quantitative Methylation-Specific PCR Analysis forMGMTPromoter Hypermethylation Detection in Gliomas

Published:2009 Issue: Volume:2009 Page:1-8
ISSN:1110-7243
Container-title:Journal of Biomedicine and Biotechnology
language:en
Short-container-title:Journal of Biomedicine and Biotechnology

Author:

Parrella Paola¹,la Torre Antonella¹,Copetti Massimiliano²,Valori Vanna M.³,Barbano Raffaela¹,Notarangelo Angelo⁴,Bisceglia Michele⁵,Gallo Antonietta Pia¹,Balsamo Teresa¹,Poeta Maria Luana⁶,Carella Massimo⁴,Catapano Domenico⁷,Parisi Salvatore⁸,Dallapiccola Bruno⁴,Maiello Evaristo³,D'Angelo Vincenzo⁷,Fazio Vito Michele¹

Affiliation:

1. Oncology Laboratory, IRCCS Casa Sollievo della Sofferenza Hospital, 71013 San Giovanni Rotondo, Italy

2. Biostatistic and Bioinformatic Unit, IRCCS Casa Sollievo della Sofferenza Hospital, 71013 San Giovanni Rotondo, Italy

3. Department of Onco-Hematology, IRCCS Casa Sollievo della Sofferenza Hospital, 71013 San Giovanni Rotondo, Italy

4. Medical Genetics Service, IRCCS Casa Sollievo della Sofferenza Hospital, 71013 San Giovanni Rotondo, Italy

5. Division of Anatomical Pathology, IRCCS Casa Sollievo della Sofferenza Hospital, 71013 San Giovanni Rotondo, Italy

6. Laboratory of Molecular Medicine and Biotechnology, Interdisciplinary Center for Biomedical Research (CIR), University Campus BioMedico, 00155 Rome, Italy

7. Department of Neurosurgery, IRCCS Casa Sollievo della Sofferenza Hospital, 71013 San Giovanni Rotondo, Italy

8. Department of Radiotherapy, IRCCS Casa Sollievo della Sofferenza Hospital, 71013 San Giovanni Rotondo, Italy

Abstract

Normal brain tissue from 28 individuals and 50 glioma samples were analyzed by real-time Quantitative Methylation-Specific PCR (QMSP). Data from this analysis were compared with results obtained on the same samples by MSP. QMSP analysis demonstrated a statistically significant difference in both methylation level (P=.000009Mann Whitney Test) and frequencies (P=.0000007, Z-test) in tumour samples as compared with normal brain tissues. Although QMSP and MSP showed similar sensitivity, the specificity of QMSP analysis was significantly higher (93%; CI95%: 84%–100%) as compared with MSP (64%; 95%CI: 46%–82%). Our results suggest that QMSP analysis may represent a powerful tool to identify glioma patients that will benefit from alkylating agents chemotherapy.

Funder

Ministero della Salute

Publisher

Hindawi Limited

Subject

Health, Toxicology and Mutagenesis,Genetics,Molecular Biology,Molecular Medicine,General Medicine,Biotechnology

Link

http://downloads.hindawi.com/journals/bmri/2009/531692.pdf

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