Comparative Hair Restorer Efficacy of Medicinal Herb on Nude (Foxn1nu) Mice

Abstract

Eclipta alba(L.) Hassk,Asiasarum sieboldii(Miq.) F. Maek (Asiasari radix), andPanax ginsengC. A. Mey (red ginseng) are traditionally acclaimed for therapeutic properties of various human ailments. Synergistic effect of each standardized plant extract was investigated for hair growth potential on nude mice, as these mutant mice genetically lack hair due to abnormal keratinization. Dried plant samples were ground and extracted by methanol. Topical application was performed on the back of nude mice daily up to completion of two hair growth generations. The hair density and length ofEclipta albatreated mice were increased significantlyP>0.001than control mice. Hair growth area was also distinctly visible inEclipta albatreated mice. On the other hand,Asiasari radixandPanax ginsengtreated mice developing hair loss were recognized from the abortive boundaries of hair coverage. Histomorphometric observation of nude mice skin samples revealed an increase in number of hair follicles (HFs). The presence of follicular keratinocytes was confirmed by BrdU labeling, S-phase cells in HFs. Therefore,Eclipta albaextract and/or phytochemicals strongly displayed incomparability of hair growth promotion activity than others. Thus, the standardizedEclipta albaextract can be used as an effective, alternative, and complementary treatment against hair loss.