Magnolin Protects against Contrast-Induced Nephropathy in Rats via Antioxidation and Antiapoptosis

Abstract

Background. Magnolin is the major active ingredient of the herbMagnolia fargesiiwhich has anti-inflammatory and antioxidative effects. Oxidative stress and apoptosis are involved in the pathogenesis of contrast-induced nephropathy (CIN). We hypothesize that Magnolin could protect against CIN through antioxidative and antiapoptotic properties.Methods. To test whether Magnolin could attenuate CIN, oxidative stress and apoptosis,in vivoandin vitro, we utilized a rat model of ioversol-induced CIN and a cell model of oxidative stress in which HK2 cells were treated with H2O2. Rats were assigned to 4 groups (n=6per group): control group, ioversol group (ioversol-induced CIN), vehicle group (CIN rats pretreated with vehicle), and Magnolin group (CIN rats pretreated with 1 mg/kg Magnolin).Results. The results showed that magnolin ameliorated the renal tubular necrosis, apoptosis, and the deterioration of renal function (P<0.05). Furthermore, Magnolin reduced the renal oxidative stress, suppressed caspase-3 activity, and increased Bcl-2 expressionin vivoandin vitro.Conclusion. Magnolin might protect CIN in rats through antioxidation and antiapoptosis.