Evaluation of Nematocidal Action against Caenorhabditis elegans of Essential Oil of Flesh Fingered Citron and Its Mechanism

Abstract

Essential oils from flesh fingered citrons were obtained by mechanical pressing extraction under optimal conditions. Thirty-three components, representing 78.25% of the total oil, were identified by gas chromatography-mass spectrometry (GC-MS). In this study, we explored the toxicity of the essential oil of flesh fingered citrons and the associated regulatory mechanism using Caenorhabditis elegans as a model organism. The mortality experiment showed that the LD50 value is 1.48 mg/ml. Oxidative stress experiments showed that essential oils of flesh fingered citrons can reduce the antioxidation of nematodes and can shorten their lifespan. In survival rate experiments for verification, d-limonene had a nematocidal effect by destroying the waterproof protective layer on the surface of the worm. β-Pinene can interfere with the normal growth of nematodes according to behavioral experiments, and reproduction experiments showed that α-pinene has very strong nematocidal properties. γ-Terpinene also has good nematocidal activity. The results demonstrated that the essential oil of flesh fingered citron has great potential to act as a new nematicide.