Green Synthesis of Silver Nanoparticles: Structural Features andIn VivoandIn VitroTherapeutic Effects againstHelicobacter pyloriInduced Gastritis

Author:

Amin Muhammad1ORCID,Hameed Sadaf1,Ali Asghar1,Anwar Farooq12,Shahid Shaukat Ali3,Shakir Imran4,Yaqoob Aqdas1,Hasan Sara1,Khan Safyan Akram5,Sajjad-ur-Rahman 6

Affiliation:

1. Department of Chemistry, University of Sargodha, Sargodha 40100, Pakistan

2. College of Pharmacy, Salman bin Abdulaziz University, Al-Kharj 11942, Saudi Arabia

3. Department of Physics, University of Agriculture, Faisalabad 38040, Pakistan

4. Deanship of Scientific Research College of Engineering, King Saud University, P.O. Box 800, Riyadh, Saudi Arabia

5. Center of Excellence in Nanotechnology Research Institute, King Fahd University of Petroleum and Minerals, Dhahran 31261, Saudi Arabia

6. Institute of Microbiology, Faculty of Veterinary Sciences, University of Agriculture, Faisalabad 38040, Pakistan

Abstract

This study evaluatesin vivoandin vitroanti-Helicobacter pylori (H. pylori)efficacy of silver nanoparticles (Ag-NPs) prepared via a cost-effective green chemistry route whereinPeganum harmalaL. seeds extract was used as a reducing and capping agent. The structural features, as elucidated by surface plasmon resonance spectrophotometry, transmission electron microscopy, and powder X-ray diffraction spectroscopy, revealed the Ag-NPs synthesized to be polydispersed in nature and spherical in shape with 5–40 nm size. A typical Ag-NPs suspension (S5), with size being 15 nm, when testedin vitroagainst forty-two local isolates and two reference strains, showed a considerable anti-H. pyloriactivity. In case ofin vivotrial againstH. pyloriinduced gastritis, after oral administration of 16 mg/kg body weight of S5for seven days, a complete clearance was recorded in male albino rates. In comparative time-killing kinetics, S5exhibited dose- and time-dependent anti-H. pyloriactivity that was almost similar to tetracycline and clarithromycin, less than amoxicillin, but higher than metronidazole. Furthermore, S5was found to be an equally effective anti-H. pyloriagent at low (≤4) and high pH with no drug resistance observed even up to 10 repeated exposures while a significant drug resistance was recorded for most of the standard drugs employed. The present results revealed the potential of the synthesized Ag-NPs as safer bactericidal agents for the treatment ofH. pyloriinduced gastritis.

Funder

King Saud University

Publisher

Hindawi Limited

Subject

Inorganic Chemistry,Organic Chemistry,Biochemistry

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