Phytoestrogensβ-Sitosterol and Genistein Have Limited Effects on Reproductive Endpoints in a Female Fish,Betta splendens

Abstract

Phytoestrogens are produced by plants and may cause endocrine disruption in vertebrates. The present study hypothesizes that phytoestrogen exposure of female Siamese fighting fish (Betta splendens) may disrupt endogenous steroid levels, change agonistic behavior expression, and potentially also disrupt oocyte development. However, only the pharmacologic dose ofβ-sitosterol had a significant effect on opercular flaring behavior, while we did not find significant effects ofβ-sitosterol or genistein on steroids or gonads. These findings are in direct contrast with previous studies on the effects of phytoestrogens in female fish. Results of the current study support previous work showing that the effects of phytoestrogen exposure may be less acute in mature femaleB. splendensthan in other fish.