In-VivoEffect of Andrographolide on Alveolar Bone Resorption Induced byPorphyromonas gingivalisand Its Relation with Antioxidant Enzymes

Abstract

Alveolar bone resorption is one of the most important facts in denture construction.Porphyromonas gingivalis(Pg) causes alveolar bone resorption, and morphologic measurements are the most frequent methods to identify bone resorption in periodontal studies. This study has aimed at evaluating the effect of Andrographolide (AND) on alveolar bone resorption in rats induced byPg. 24 healthy maleSprague Dawleyrats were divided into four groups as follows: normal control group and three experimental groups challenged orally withPgATCC 33277 five times a week supplemented with 20 mg/kg and 10 mg/kg of AND for twelve weeks. Alveolar bones of the left and right sides of the mandible were assessed by a morphometric method. The bone level, that is, the distance from the alveolar bone crest to cementumenamel junction (CEJ), was measured using 6.1 : 1 zoom stereomicroscope and software. AND reduced the effect ofPgon alveolar bone resorption and decreased the serum levels of Hexanoyl-Lysine (HEL); furthermore the reduced glutathione/oxidised glutathione (GSH/GSSG) ratio in AND treated groups (10 and 20 mg/kg) significantly increased when compared with thePggroup(P<0.05). We can conclude that AND suppresses alveolar bone resorption caused byPgin rats.