CTGF Attenuates Tendon-Derived Stem/Progenitor Cell Aging-Reference-Cited by-同舟云学术

CTGF Attenuates Tendon-Derived Stem/Progenitor Cell Aging

Published:2019-11-11 Issue: Volume:2019 Page:1-12
ISSN:1687-966X
Container-title:Stem Cells International
language:en
Short-container-title:Stem Cells International

Author:

Rui Yun-feng¹²³⁴⁵^ORCID,Chen Min-hao¹²³⁴⁵^ORCID,Li Ying-juan⁵⁶^ORCID,Xiao Long-fei¹²³⁴^ORCID,Geng Peng¹²³⁴^ORCID,Wang Pei⁴^ORCID,Xu Zheng-yuan⁴^ORCID,Zhang Xuan-pu⁴^ORCID,Dai Guang-chun¹²³⁴⁵^ORCID

Affiliation:

1. Department of Orthopaedics, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, China

2. Orthopaedic Trauma Institute, Southeast University, Nanjing, China

3. Trauma Center, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, China

4. School of Medicine, Southeast University, Nanjing, China

5. China Orthopedic Regenerative Medicine Group, Hangzhou, Zhejiang 310000, China

6. Department of Geriatrics, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, China

Abstract

Aged tendon-derived stem/progenitor cells (TSPCs) lead to age-related tendon disorders and impair tendon healing. However, the underlying molecular mechanisms of TSPC aging remain largely unknown. Here, we investigated the role of connective tissue growth factor (CTGF) in TSPC aging. CTGF protein and mRNA levels were markedly decreased in the aged TSPCs. Moreover, recombinant CTGF attenuates TSPC aging and restores the age-associated reduction of self-renewal and differentiation of TSPCs. In addition, cell cycle distribution of aged TSPCs was arrested in the G1/S phase while recombinant CTGF treatment promoted G1/S transition. Recombinant CTGF also rescued decreased levels of cyclin D1 and CDK4 and reduced p27kip1 expression in aged TSPCs. Our results demonstrated that CTGF plays a vital role in TSPC aging and might be a potential target for molecular therapy of age-related tendon disorders.

Funder

Six Projects Sponsoring Talent Summits of Jiangsu Province

Publisher

Hindawi Limited

Subject

Cell Biology,Molecular Biology

Link

http://downloads.hindawi.com/journals/sci/2019/6257537.pdf

Reference42 articles.

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2. Current concepts in the management of tendon disorders

3. Use of autologous growth factors in aging tendon and chronic tendinopathy