Association between RAS Gene Polymorphisms (ACE I/D, AGT M235T) and Henoch-Schönlein Purpura in a Turkish Population

Abstract

Henoch-Schönlein purpura (HSP) is a small-vessel vasculitis of autoimmune hypersensitivity, and renin-angiotensin system (RAS) regulates vascular homeostasis and inflammation with activation of cytokine release. Thus, we aimed to investigate the association between HSP andACE I/DandAGT M235Tpolymorphisms. Genotyping was determined by allele specific PCR and PCR-RFLP. We obtained a significant difference in genotype distribution (p= 0.003) and allele frequencies (p< 0.001) ofACE I/Dpolymorphism between patients and controls, while no significant association was detected in genotype distribution (p> 0.05) and allele frequencies (p> 0.05) of theAGT M235Tpolymorphism. Risk assessment showed significant risk for HSP in the subjects both with the ID + DD genotype (p= 0.019, OR: 2.288, 95% CI: 1.136–4.609) and D allele (OR: D vs. I: 2.0528, 95% CI: 1.3632–3.0912,p= 0.001) while no significant risk was obtained for HSP in the subjects both with the MT + TT genotype (p= 0.312, OR: 1.3905, 95% CI: 0.7326–2.6391) and T allele (OR: T vs. M: 1.065, 95% CI: 0.729–1.557,p= 0.743). Furthermore, when patients were stratified by the presence of certain systemic complications of HSP, no significant association was detected withACE I/D, andAGT M235Tpolymorphisms. Our findings suggest thatACE I/Dpolymorphism is significantly associated with HSP susceptibility.