Histochemical Investigation and Kinds of Alkaloids in Leaves of Different Developmental Stages inThymus quinquecostatus

Author:

Jing Haiting1,Liu Jing2,Liu Hanzhu1,Xin Hua13

Affiliation:

1. College of Life Sciences, Qingdao Agricultural University, Qingdao 266109, China

2. Central Laboratory, Qingdao Agricultural University, Qingdao 266109, China

3. University Key Laboratory of Plant Biotechnology, Shandong, Qingdao 266109, China

Abstract

Thymus quinquecostatus, with more medical value, is a kind of wild plants. In order to exploit and utilize this plant, we studied the species and locations of alkaloids in its leaves. In this paper, histochemical study of leaves at different developing stages was taken to localize the alkaloids. Meanwhile, the kinds and content of alkaloids in leaves were identified using GC-MS technique. It was found that there were two kinds of glandular trichomes, namely, peltate trichomes and capitate trichomes, on the surface of leaves, and their secretory cells could secrete alkaloids. Results showed that trichomes could secrete alkaloids as soon as the first pair of leaves formed, and there were altogether 18 kinds of alkaloids identified by GC-MS. Nearly all of these alkaloids of leaves at different developing stages were distinct from each other, except one, 3-methoxy-a-methyl-benzeneethanamine, persists at different developing stages with high concentration.

Funder

Natural Science Foundation of Shandong Province

Publisher

Hindawi Limited

Subject

General Environmental Science,General Biochemistry, Genetics and Molecular Biology,General Medicine

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