Isolation, Identification, and Susceptibility Profile of E. coli, Salmonella, and S. aureus in Dairy Farm and Their Public Health Implication in Central Ethiopia

Abstract

A cross-sectional study was conducted from November 2018 to May 2019 in Bishoftu and Dukem in central part of Ethiopia. The objectives of the present study were to isolate and identify S. aureus, E. coli, and Salmonella from dairy cattle, personnel, and equipment at farms. In addition to this, antimicrobial resistance profiles of the isolates were determined. A total of 607 samples consisting of fresh cow milk (125), fecal sample (211), nasal swab (211), pooled milkers’ hand swabs (20), pooled floor swabs (20), and tank milk (20) samples were collected from 20 dairy farms, which included 211 animals. Structured questionnaire was designed and administered to dairy farm owners and dairy food consumers to assess their consumption behavior and antibiotics usage. The samples were examined for the presence of S. aureus, E. coli, and Salmonella following standard techniques and procedures outlined by the International Organization for Standardization. Subsequently, 62 (15.7%) of S. aureus were isolated from 396 of the totals analyzed samples for S. aureus. Out of the 62 isolated S. aureus, 35/211(16.7%), 19/125(15.2%), 6/20(30%), 2/20(10%), and 0/20(0%) were from nasal swabs, udder milk, bulk milk, pooled hand swab, and floor swabs, respectively. On the other hand, 30 (7.6%) of E. coli were isolated from 396 of the totals analyzed samples for E. coli. Out of the 30 isolated E. coli, 17/211(8.1%), 12/125(9.6%), 0/20(0%), 0/20(0%), and 1/20(5%) were from faeces, udder milk, bulk milk, pooled hand swab, and floor swabs, respectively. In line with this, 13 (4.8%) of Salmonella were isolated from 271 of the totals analyzed samples for Salmonella. Out of the 13 isolated Salmonella, 10/211(4.7%), 2/20(10%), 0/20(0%), and 1/20(5%) were from faeces, bulk milk, pooled hand swab, and floor swabs, respectively. Subsequently, 62 of S. aureus, 30 of E. coli, and 13 of Salmonella isolates were subjected to antimicrobial susceptibility testing, and all isolates were resistant to at least one or more antimicrobials tested. Penicillin, methicillin, and trimethoprim/sulfamethoxazole are drugs to which a large proportion of isolated S. aureus were highly resistant, which range from 90% to 100%. From 30 tested E. coli, they showed (83%) resistance to Tetracycline and 80% to Vancomycin. The resistance level of 13 isolated Salmonella was 69% to Nalidixic acid and 54% to Vancomycin. Multiple drug resistance was detected in high (98.4%) for S. aureus, (56.7%) for E. coli, and (53.9%) for Salmonella. High proportion of multiple drug resistant in the dairy farm alerts concern for animal and public health as these drugs are used widely for treatment and prophylaxis in animals and humans.