The Osteogenesis of Bone Marrow Stem Cells on mPEG-PCL-mPEG/Hydroxyapatite Composite Scaffold via Solid Freeform Fabrication

Author:

Liao Han-Tsung12,Chen Yo-Yu3ORCID,Lai Yu-Ting12ORCID,Hsieh Ming-Fa4ORCID,Jiang Cho-Pei5

Affiliation:

1. Department of Plastic and Reconstructive Surgery, Craniofacial Research Center, Chang Gung Memorial Hospital, College of Medicine, Chang Gung University, Kweishan, Taoyuan 333, Taiwan

2. Department of Chemical and Materials Engineering, Chang Gung University, Kweishan, Taoyuan 333, Taiwan

3. Institute of Mechanical and Electro-Mechanical Engineering, National Formosa University, Yunlin County 632, Taiwan

4. Department of Biomedical Engineering, Chung Yuan Christian University, 200 Chung Pei Road, Chung Li 32023, Taiwan

5. Department of Power Mechanical Engineering, National Formosa University, Yunlin County 632, Taiwan

Abstract

The study described a novel bone tissue scaffold fabricated by computer-aided, air pressure-aided deposition system to control the macro- and microstructure precisely. The porcine bone marrow stem cells (PBMSCs) seeded on either mPEG-PCL-mPEG (PCL) or mPEG-PCL-mPEG/hydroxyapatite (PCL/HA) composite scaffold were cultured under osteogenic medium to test the ability of osteogenesisin vitro. The experimental outcomes indicated that both scaffolds possessed adequate pore size, porosity, and hydrophilicity for the attachment and proliferation of PBMSCs and the PBMSCs expressed upregulated genes of osteogensis and angiogenesis in similar manner on both scaffolds. The major differences between these two types of the scaffolds were the addition of HA leading to higher hardness of PCL/HA scaffold, cell proliferation, and VEGF gene expression in PCL/HA scaffold. However, thein vivobone forming efficacy between PBMSCs seeded PCL and PCL/HA scaffold was different from thein vitroresults. The outcome indicated that the PCL/HA scaffold which had bone-mimetic environment due to the addition of HA resulted in better bone regeneration and mechanical strength than those of PCL scaffold. Therefore, providing a bone-mimetic scaffold is another crucial factor for bone tissue engineering in addition to the biocompatibility, 3D architecture with high porosity, and interpored connection.

Funder

National Science Council

Publisher

Hindawi Limited

Subject

General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

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