Redox Control of Antioxidant and Antihepatotoxic Activities ofCassia surattensisSeed Extract against Paracetamol Intoxication in Mice: In Vitro and In Vivo Studies of Herbal Green Antioxidant

Abstract

The therapeutic potential ofCassia surattensisin reducing free radical-induced oxidative stress and inflammation particularly in hepatic diseases was evaluated in this study. The polyphenol richC. surattensisseed extract showed good in vitro antioxidant.C. surattensisseed extract contained total phenolic content of 100.99 mg GAE/g dry weight and there was a positive correlation (r>0.9) between total phenolic content and the antioxidant activities of the seed extract.C. surattensisseed extract significantly (p<0.05) reduced the elevated levels of serum liver enzymes (ALT, AST, and ALP) and relative liver weight in paracetamol-induced liver hepatotoxicity in mice. Moreover, the extract significantly (p<0.05) enhanced the antioxidant enzymes and glutathione (GSH) contents in the liver tissues, which led to decrease of malondialdehyde (MDA) level. The histopathological examination showed the liver protective effect ofC. surattensisseed extract against paracetamol-induced histoarchitectural alterations by maximum recovery in the histoarchitecture of the liver tissue. Furthermore, histopathological observations correspondingly supported the biochemical assay outcome, that is, the significant reduction in elevated levels of serum liver enzymes. In conclusion,C. surattensisseed extract enhanced the in vivo antioxidant status and showed antihepatotoxic activities, which is probably due to the presence of phenolic compounds.