Effect ofHelicobacter pyloriEradication on TLR2 and TLR4 Expression in Patients with Gastric Lesions

Author:

Cadamuro Aline Cristina Targa1ORCID,Rossi Ana Flávia Teixeira1,Matos Biselli-Périco Joice1,Fucuta Pereira Patrícia2,Do Vale Edla Polsinelli Bedin Mascarin2,Acayaba Ricardo3,Leite Kátia Ramos Moreira4,Goloni-Bertollo Eny Maria5,Silva Ana Elizabete1ORCID

Affiliation:

1. Department of Biology, São Paulo State University (UNESP), São José do Rio Preto Campus, Rua Cristóvão Colombo 2265, 15054-000 São José do Rio Preto, SP, Brazil

2. Base Hospital, Ambulatory of Gastrohepatology, Avenida Brigadeiro Faria Lima 5544, 15090-000 São José do Rio Preto, SP, Brazil

3. João Paulo II Hospital, Rua Doutor Eduardo Nielsem 960, 15030-070 São José do Rio Preto, SP, Brazil

4. School of Medicine, São Paulo University (USP), Avenida Dr. Arnaldo 455, 01246-903 São Paulo, SP, Brazil

5. School of Medicine, FAMERP, Avenida Brigadeiro Faria Lima 5416, 15090-000 São José do Rio Preto, SP, Brazil

Abstract

Objective.Helicobacter pylori(Hp) is recognized by TLR4 and TLR2 receptors, which trigger the activation of genes involved in the host immune response. Thus, we evaluated the effect of eradication therapy on TLR2 and TLR4 mRNA and protein expression inH. pylori-infected chronic gastritis patients (CG-Hp+) and 3 months after treatment.Methods. A total of 37 patients CG-Hp+ were evaluated. The relative quantification (RQ) of mRNA was assessed by TaqMan assay and protein expression by immunohistochemistry.Results. Before treatment bothTLR2andTLR4mRNA in CG-Hp+ patients were slightly increased (TLR2= 1.32;TLR4= 1.26) in relation to Hp-negative normal gastric mucosa (P0.05). After successful eradication therapy no significant change was observed (TLR2= 1.47;TLR4= 1.53;P>0.05). In addition, thecagAandvacAbacterial genotypes did not influence the gene expression levels, and we observed a positive correlation between the RQ values ofTLR2andTLR4, both before and after treatment. Immunoexpression of the TLR2 and TLR4 proteins confirmed the gene expression results.Conclusion. In conclusion, the expression of bothTLR2andTLR4is increased in CG-Hp+ patients regardless ofcagAandvacAstatus and this expression pattern is not significantly changed after eradication of bacteria, at least for the short period of time evaluated.

Funder

São Paulo Research Foundation

Publisher

Hindawi Limited

Subject

Cell Biology,Immunology

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