Hyal1 Expression in Colorectal Carcinoma Cell Migration and Invasiveness: Significance and Mechanism

Abstract

Objective. To clarify the significance of hyaluronidsase 1 (Hyal1) expression in colorectal carcinoma (CRC) and its impact on tumor cell migration and invasiveness. Methods. Human CRC cell lines SW480, HCT116, and SW620 were purchased, ELISA and western blot were used to detect the expression of Hyal1 in cells, CCK-8 assay to detect cell proliferation ability, cell scratch assay to check cell migration rate, and cell invasion was detected by the transwell assay. The correlation of Hyal1 with CRC cell migration and invasiveness capacities was analyzed. Result. ELISA results showed that supernatant Hyal1 level was the lowest in SW480, highest in HCT116, with the level in SW620 in between ( $P<0.05$ ). No evident difference was identified by western blot in Hyal1 protein expression among the three cells ( $P>0.05$ ). The cell scratch assay and transwell assay showed that the migration and invasion ability of HCT116 cells was higher than that of SW620 ( $P<0.05$ ). In vitro, Hyal1 had a synergistic relationship with the invasiveness and migration capacities of CRC cells ( $P<0.05$ ). Conclusion. Hyal1 is elevated in CRC and is consistent with the invasiveness and metastasis abilities of CRC cells. It is hoped that this research can provide reference for future prevention and treatment of CRC.