Genes Encoding Toxin ofClostridium difficilein Children with and without Diarrhea-Reference-Cited by-同舟云学术

Genes Encoding Toxin ofClostridium difficilein Children with and without Diarrhea

Published:2014 Issue: Volume:2014 Page:1-4
ISSN:2090-908X
Container-title:Scientifica
language:en
Short-container-title:Scientifica

Author:

Merino Victor R. C.¹,Nakano Viviane¹,Finegold Sydney M.²³⁴,Avila-Campos Mario J.¹

Affiliation:

1. Anaerobe Laboratory, Department of Microbiology, Institute of Biomedical Science, University of São Paulo, São Paulo, SP, Brazil

2. Veterans Affairs, West Los Angeles Medical Center, Los Angeles, CA, USA

3. Department of Medicine, School of Medicine, University of California, Los Angeles, CA, USA

4. Department of Microbiology, Immunology and Molecular Genetics, School of Medicine, University of California, Los Angeles, CA, USA

Abstract

The presence of gene 16S rRNA and genes encoding toxin A (tcdA), toxin B (tcdB), and binary toxin (cdtA/cdtB) ofClostridium difficilein stool samples from children with (110) and without (150) diarrhea was determined by using a TaqMan system. Fifty-seven (21.9%) out of 260 stool samples harbored the 16S rRNA gene. The genetic profile oftcdA+/tcdB−andcdtA+/cdtB+was verified in oneC. difficile-positive diarrhea sample and oftcdA+/tcdB+in threeC. difficile-positive nondiarrhea samples. The presence oftcdA+/tcdB+in stools obtained from children without diarrhea, suggests that they were asymptomatic carriers of toxigenic strains.

Funder

Fundação de Amparo a Pesquisa do Estado de Sao Paulo

Publisher

Hindawi Limited

Subject

General Agricultural and Biological Sciences,General Environmental Science

Link

http://downloads.hindawi.com/journals/scientifica/2014/594014.pdf

Reference17 articles.

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