Affiliation:
1. Department of Radiation Oncology, The Second Hospital of Jilin University, Changchun, 130021, China
Abstract
Radioresistance of breast cancer is a major reason for therapeutic failure and limits further increases in the dose of radiation due to severe adverse effects. Recently, long noncoding RNAs (lncRNAs) have been shown to regulate cancer proliferation, chemoresistance, and radioresistance. Among these lncRNAs, lncRNA GAS5 expression was shown to be downregulated in breast cancer and related to trastuzumab resistance. However, its role in the radiation response is unclear. In this study, we demonstrated that lncRNA GAS5 expression was reduced in irradiated cells and that overexpression of GAS5 reduced cell viability and promoted cell apoptosis after irradiation. Moreover, overexpression of GAS5 resulted in increased G2/M arrest and unrepaired DNA damage, indicating a radiosensitizing role of GAS5 in breast cancer cells. Finally, we found that a GAS5-interacting miRNA, miR-21, reversed the radiosensitizing effects of GAS5 by inhibiting the apoptotic pathway. In conclusion, we found that lncRNA GAS5 sensitized breast cancer cells to ionizing radiation by inhibiting DNA repair and suppressing miR-21, identifying novel targets for breast cancer radiosensitization.
Subject
General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine
Reference29 articles.
1. Radiotherapy and breast cancer;K. J. Mulvany;South African Nursing Journal,2014
2. Radiotherapy and breast cancer: recent developments;B. Cutuli;Sein,2003
3. Recurrence rates after treatment of breast cancer with standard radiotherapy with or without additional radiation;H. Bartelink;New England Journal of Medicine,2001
4. Emerging roles of long non-coding RNA in cancer
5. Progress of long non-coding RNA in regulating cell autophagy in cancer;W. W. Liu;Chinese Journal of Oncology,2018
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