Development of a quantitative and conformation-sensitive ATR-FTIR biosensor for Alzheimer's disease: The effect of deuteration on the detection of the Aβ peptide

Author:

Kleiren Emilie1,Ruysschaert Jean-Marie1,Goormaghtigh Erik1,Raussens Vincent12

Affiliation:

1. Center for Structural Biology and Bioinformatics, Laboratory of Structure and Function of Biological Membranes, Université Libre de Bruxelles, Brussels, Belgium

2. Laboratory of Structure and Function of Biological Membranes, Université Libre de Bruxelles, Campus Plaine CP 206/02, Blvd du Triomphe, Acces 2, B1050 Brussels, Belgium

Abstract

Alzheimer's disease (AD) is the most common form of dementia worldwide and represents a growing socio-economical issue. To date, no reliable diagnosis can be obtained at an early-stage of the disease, though it is now recognized that the aggregation of the amyloid β (Aβ) peptide is responsible for the onset of the disease. Recent studies have shown that soluble amyloid oligomers present in the physiological fluids were the most neurotoxic species and correlated best with the first signs of cognitive decline, which makes them good biomarkers in the development of a diagnostic tool.We describe here a new type of biosensor, based on attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy, that would be able to specifically detect and quantify the presence of the different forms of the Aβ peptide in solution. The principle of the detection relies on the recognition of the peptides by specific antibodies that were previously grafted on the surface of an ATR element, consisting of a functionalized germanium crystal. We show that the BIA-ATR technology is able to detect the presence of Aβ if incubated in deuterated water and that this step is crucial in the development of our conformation-sensitive biosensor for AD.

Funder

Interuniversity Attraction Poles

Publisher

Hindawi Limited

Subject

Spectroscopy

Cited by 15 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Analysis of Biomolecular Dynamics Under Fourier Transform Infrared Spectroscopy;Optical Spectroscopic and Microscopic Techniques;2022

2. Ready-to-Use Germanium Surfaces for the Development of FTIR-Based Biosensors for Proteins;Langmuir;2020-10-02

3. Body fluid analysis;Vibrational Spectroscopy Applications in Biomedical, Pharmaceutical and Food Sciences;2020

4. Stress Biomarkers in Biological Fluids and Their Point-of-Use Detection;ACS Sensors;2018-09-28

5. Infrared imaging of high density protein arrays;The Analyst;2017

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