Abundance and Significance of Neuroligin-1 and Neurexin II in the Enteric Nervous System of Embryonic Rats

Author:

Wang Dongming1,Pan Jingnian2,Song Guoxin3,Gao Ni1,Zheng Yi1,Zhang Qiangye1,Li Aiwu1ORCID

Affiliation:

1. Department of Pediatric Surgery, Qilu Hospital, Shandong University, Shandong, China

2. Internal Medicine, Qingzhou Clinical School, Weifang Medical College, Weifang, China

3. Department of Pediatric Surgery, Weihai Municipal Hospital, Shandong, China

Abstract

Aim. To investigate the abundance of neuroligin-1 and neurexin II in the enteric nervous system (ENS) of rats on different embryonic days and to explore their potential significance.Methods. The full-thickness colon specimens proximal to the ileocecal junction of rats on embryonic days 16, 18, and 20 and of newborns within 24 hours (E16, E18, E20, and Ep0) were studied, respectively. qRT-PCR was applied for detecting the expressions of neuroligin-1 and neurexin II on mRNA, and western blotting was employed for detecting their further expressions on the whole tissue. Finally, the histological appearance of neuroligin-1 and neurexin IIαwas elucidated using immunohistochemical staining.Results. qRT-PCR showed that the neuroligin-1 and neurexin II mRNA expressions of groups E16, E18, E20, and Ep0 increased gradually with the growth of embryonic rats (P<0.05). Western blotting confirmed the increasing tendency. In immunohistochemical staining, proteins neuroligin-1 and neurexin IIαpositive cells concentrated mostly in the myenteric nerve plexus of the colon and their expressions depend on the embryonic time.Conclusion. Neuroligin-1 and neurexin II were both expressed in the ENS and have temporal correlation with the development of ENS, during which neuronal intestinal malformations (NIM) may occur due to their disruptions and consequent abnormal ENS development.

Funder

National Natural Science Foundation of China

Publisher

Hindawi Limited

Subject

General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

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